MTBE

Bruce Roe BROE at aardvark.ucs.ou.edu
Tue Jan 18 10:16:16 EST 2000


Happy y2k,

	MTBE aka Modified Tris-borate-EDTA buffer, is made by
		doubling the Tris and halfing the borate from
		what was in typical TBE when it was initially
		introduced.  ABI recommends slightly different
		amounts and we've pretty much stuck with what
		ABI recommends for the 377's

	10x MTBE (Modified Tris-borate-EDTA buffer
		1.3 M Tris, 0.4 M Boric acid, and 0.02 M EDTA
		in double distilled water. 

                162 g           Tris base
                27.5 g          Boric acid
                9.3 g           Na2EDTA
                ddH2O to 1 L

	10X ABI TBE: 
                216 g  Tris base	108 g
                110 g  boric acid	 55 g
                16.6 g EDTA	       	8.3 g
        	Add ddwater to 2 L 	Add ddwater to 1 L


	If you don't know about our online protocol book, it's
available at URL: http://www.genome.ou.edu/proto.html

Enjoy,
--b.roe
*************************************************************************
Bruce A. Roe, Ph.D  George Lynn Cross Research Professor
                    Department of Chemistry and Biochemistry
                    University of Oklahoma, Norman, OK 73019-0370, U.S.A.
Phone: (405) 325-4912 or 7610;  FAX: (405) 325-7762;  e-mail: broe at ou.edu
********************** http://www.genome.ou.edu/ ************************

 You write: 
=> Date: Mon, 17 Jan 2000 22:57:12 +0000
=> From: Mathew Nightingale <mathew at mufc.junglelink.co.uk>
=> Subject: MTBE
=> 
=> Hi Everyone,
=> 
=> Hope you all had a good Christmas and New Year.
=> 
=> I am writing to ask if anyone has ever used MTBE buffer apparently it
=> increases the read length for DNA sequencing.
=> 
=> I hope that someone can shed some light on this and maybe offer a protocol
=> to try.
=> 
=> Thanks in advance
=> 
=> Mathew Nightingale
=> 
=> 
=> 


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