could someone point me to literature outlining the current status of
direct genomic sequencing? I've been out of the sequencing loop for the
last couple of years and would like to know if there are reliable
protocols being used to generate sequence data from genomic DNA using
gene specific primers. We are interested in determining the allele of a
particular gene in a large number of bacterial isolates. We were
leaning towards PCR and hybridization with allele-specific probes but
someone mentioned that we might want to try direct sequencing instead
and we are definitely intrigued. What are people's views on this ?
thanks in advance,
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