Loading dyes

Mr.D.J.Cain dcain at hgmp.mrc.ac.uk
Sat Jul 8 14:49:07 EST 2000


Scottie, 

Check out www.microzone.co.uk I know they sell a buffer with the pink dye
and are a lot cheaper than the 20 pounds per ml that Amersham wanted last
time I looked.

David Cain

On 7 Jul 2000, Scottie Adams wrote:

> Greetings
> 
>  For a while I was using Amersham kits for DNA sequencing and they include
> a nice gel loading buffer with a pink dye in it.  I eventually swiched back
> to PE Taq FS - but kept using the   Amersham loading dye.  Now we are out
> of dye and  of course it only comes in the  Amersham sequencing kit.  Does
> any one know what dye it might be - or any other dye I can spike into my
> loading buffer to make loading easier.  My tech is spoiled and has never
> loaded  samples without dye.
> 
> Thanks
> 
> Scottie
> 
> Scottie Adams aka Pamela Scott Adams
> 
> Manager
> Molecular Biology Core Facility
> Trudeau Institute
> 100 Algonquin Avenue
> Saranac Lake, NY    12983
> Phone:  518-891-3080, Ext. 115
> Fax:    518-891-5126
> Email: sadams at northnet.org
> sadams at trudeauinstitute.org
> http://www.trudeauinstitute.org
> 
> 
> 
> ---
> 








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