Moran at wadsworth.org
Tue Jul 11 08:31:51 EST 2000
We make our own loading dye based on the PE formula...as you probably know, it
is blue. The composition is 25 mM EDTA, pH 8.0, containing 50mg/ml Blue
Dextran 2000. I hope this is helpful. Of course, if it is pink you
NYS Dept of Health
Molecular Genetics Core
moran at wadsworth.org.
In article <8k80fu$bcs$1 at mercury.hgmp.mrc.ac.uk>, sadams at northnet.org says...
> For a while I was using Amersham kits for DNA sequencing and they include
>a nice gel loading buffer with a pink dye in it. I eventually swiched back
>to PE Taq FS - but kept using the Amersham loading dye. Now we are out
>of dye and of course it only comes in the Amersham sequencing kit. Does
>any one know what dye it might be - or any other dye I can spike into my
>loading buffer to make loading easier. My tech is spoiled and has never
>loaded samples without dye.
>Scottie Adams aka Pamela Scott Adams
>Molecular Biology Core Facility
>100 Algonquin Avenue
>Saranac Lake, NY 12983
>Phone: 518-891-3080, Ext. 115
>Email: sadams at northnet.org
>sadams at trudeauinstitute.org
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