Preparation of Template for Sequencing

Pezzolesi, Marcus Marcus.Pezzolesi at joslin.harvard.edu
Tue Jul 11 17:24:34 EST 2000


We have recently been having problems with our cycle sequencing and feel
that the problem lies in the preparation of DNA template for sequencing.  In
the past, we were having quite a bit of success with cutting bands from
agarose and then using Qiagens Qiaquick spin columns, but this is no longer
working well. We have also tried PEG precipitation, but also with mixed
results. 

Can anyone offer a protocol which has proven to work well?

Thanks.


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