Preparation of Template for Sequencing

Mr.M.R.M.Botcherby mrbotche at hgmp.mrc.ac.uk
Wed Jul 12 03:53:06 EST 2000


This works (honest!)
Cut out band, place in Eppendorf tube with 40 ul of water, leave in fridge 
overnight.  The resulting eluent makes perfect sequencing template...

In article <8khatf$gss$1 at mercury.hgmp.mrc.ac.uk>,
	"Pezzolesi, Marcus" <Marcus.Pezzolesi at joslin.harvard.edu> writes:
> We have recently been having problems with our cycle sequencing and feel
> that the problem lies in the preparation of DNA template for sequencing.  In
> the past, we were having quite a bit of success with cutting bands from
> agarose and then using Qiagens Qiaquick spin columns, but this is no longer
> working well. We have also tried PEG precipitation, but also with mixed
> results. 
> 
> Can anyone offer a protocol which has proven to work well?
> 
> Thanks.
> 
> 
> ---
> 
> 

-- 
Marc Botcherby
Mouse Sequencing Group leader UK-HGMP
m.botcherby at hgmp.mrc.ac.uk
Tel:01223 494555 Fax: 01223 494512








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