To anyone who can help
Our laboratory has recently changed from using the Rhod.Kit to
using the Big Dye Terminator kit. Despite the raw data looking
more than adequate, base calling does not begin until
approximately 100bp after the primer peak. This probelm only
began with the Big Dye kit. I don't want to go through all my
sequences and have to correct by hand (obviously this will take
considerable length of time).
Has anyone else had this problem and if so, could you please let
me know how you corrected it.
medplr at leeds.ac.uk