Question for your collective minds:
Yesterday, our 377-96 terminated a 7 hour run after 3.5 hours for no
apparent reason. The gel image was created and the sequence looked
beautiful...to about 300 bases. Realizing that I needed about 50 more
bases to form my contigs, I started the machine again and got what
appeared to be sequence characteristic of hours 3.5-6.5 of a usual 1200
scan/hr run. However, I couldn't get Seq. Anal. 3.4 to decipher the data
from the latter run in any meaningful way (runs of 20 thymadines, 15
adenosines, etc. were common). Now, I think that if I can merge the two
gel files into one mega-file, the analysis program should be able to
process the data properly. Does anyone know how I might be able to do
this?
Due to the nature of the samples, simply redoing the reactions isn't possible.
Thanks in advance,
dave
--
David O'Connor
dhoconno at students.wisc.eduhttp://www.sit.wisc.edu/~dhoconno
Dept. of Medical Microbiology and Immunology
University of Wisconsin
