sequencer comparison

Bruce Roe BROE at aardvark.ucs.ou.edu
Thu Nov 2 09:24:12 EST 2000


James,
        Since you already have extensive experience with the 377
and obtaining high quality sequencing templates for this slab
gel instrument, I would strongly advise in favor of obtaining
another 377.  You can pick up a used one for ~$30,000 and could
use your existing template isolation protocols, etc.  Switching
to a capillary instrument will require much additional care in
isolating very clean templates, in addition to the high cost of
the instrument, significant amount of down time for the 3700,
and extreme sensitivity to contaminants in obtaining successful
reads with the capillary instruments.
        That's my 2 cents worth.
Cheers,
--bruce
*************************************************************************
Bruce A. Roe, Ph.D  George Lynn Cross Research Professor
                    Department of Chemistry and Biochemistry
                    University of Oklahoma, Norman, OK 73019-0370, U.S.A.
Phone: (405) 325-4912 or 7610;  FAX: (405) 325-7762;  e-mail: broe at ou.edu
********************** http://www.genome.ou.edu/ ************************

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=> Date: Wed, 01 Nov 2000 23:08:31 +0000
=> From: "James M.Schupp" <James.Schupp at NAU.EDU>
=> Subject: sequencer comparison
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=> Newsgroups: bionet.genome.autosequencing
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=> 
=> Hello all,
=> 
=> 	Looking for some advice on purchasing an automated 
=> sequencer/genotyper.  We are a small one ABI 377 lab looking to add a 
=> second machine, thinking about the ABI3100 16 capillary machine.  Anyone 
=> have some feedback on this machine, the megabace 1000 (there is a 16 and a 
=> 48 capillary machine available from APBiotech now) and the MJ Research 
=> BaseStation? Concerned about throughput, machine downtime and consumables 
=> cost.  Not considering the LiCor machine due to different chemistries.  Any 
=> feedback would be very useful. Thanks
=> 
=> Jim Schupp
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=> 
=> James M. Schupp
=> Project Director
=> Biology Department, Box 5640
=> Northern Arizona University
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