BigDye & FAM-Primer ?

Carsten Hohoff hohoff at uni-muenster.de
Tue Nov 7 06:14:03 EST 2000


Does anyone from this list has experience in using a
fluorescently-labelled PCR product for sequencing with the BigDye kit ? 

Will the label (e.g., 6-FAM) interfere with FRET process of the labelle dye
terminators ?

The idea is that I analyze the fragment length of this PCR
product (only one allele)  first and then use it for cycle sequencing
without the necessity to change the fluorescently-labelled primer by a non
labelled primer in a further PCR.

Any constructive comment is greatly appreciated

Carsten Hohoff

Dept of Legal Medicine
Muenster
Germany 


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