3700: when to chenge the array?

Phillip San Miguel pmiguel at purdue.edu
Mon Nov 20 07:56:59 EST 2000


Barbara Simionati wrote:

> Hi,
>
> We have a 3700 and our first array has done more than 500 runs.
> I would like to know when you recognize that it is time to change it.
> What kind of signs do you consider? bad sequence quality (large peaks),
> no sequence (capillary occlusion).
> Does the problem always occur in the same capillary in consecutive rus?
> [...]

    We've gotten over 900 runs on our current array--which is our first. Until last week none of the
capillaries were blocked. We frequently did 104 samples per run. But last week several caps clogged
when buffer ran low in the load block during a batch of runs. I still have hopes of recovering these
caps though. Because months ago our 3700 froze in mid-loading (loading block dry and oven doors
open) and I didn't discover this until the next morning. Nevertheless, a POP6towater and
InstallArray (from service tools) resurrected the array. (Actually POP6towater and InstallArray are
just components of RegenerateArray.)
    This doesn't answer your question. But I thought you might like to know it is possible for an
array to continue to work long after its "spec" 300 runs. I've heard there is (was?) and array at
Celera that lasted 2500 runs.
Phillip San Miguel
Purdue Genomics Core Facility




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