'Half BD' big dye diluent

Dr N.I.Leaves nleaves at hgmp.mrc.ac.uk
Tue Nov 21 07:35:23 EST 2000


I have only ever used 10ul rxns with 4ul of BD. But in my previous job we
also routinely replaced 1/2 of the BD with half BD from GenPak, meaning
only 2ul of BD/rxn! We always loaded all of the sample and we were using
373 and 377.

I am currently running 3700 machines and have not experimented with half
BD yet and cannot tell you that it will work. However, it does
seem that these machine are even more sensitive and one might guess that 
you could get away with even less BD reagent. 

Good luck

nick leaves


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************************************************** 
Dr N I Leaves
Mouse Sequencing
MRC HGMP Resource Centre 
Hinxton 
Cambridge CB10 1SB 
tel: 01223 494557 (office) or 01223 494541 (lab)
email: nleaves at hgmp.mrc.ac.uk
**************************************************

On 15 Nov 2000, Kevin Clark wrote:

> Has anyone tried the diluents on the market that you can add to BigDye
> to reduce the amount of sequencing kit you use?
> 
> Are they any good?
> 
> How little BD kit can you use and does it reduce read lengths?
> 
> Thanks
> 
> Kevin
> 
> 
> ---
> 
> 
> 








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