t.tarragona at ic.ac.uk
Mon Oct 9 06:02:37 EST 2000
I use Sequencher for the Mac and is very good. I notice that they have also
produce a version for Windows, worth having a look at www.genecodes.com.
"Sharon R. Minnerath" <minne002 at maroon.tc.umn.edu> wrote in message
news:8qo5pe$6p8$1 at mercury.hgmp.mrc.ac.uk...
> Hello Everyone,
> I have a question about Alignment Software. I am currently sequencing a
> BAC which I am told is about 340 kb. I have it nebulized and subcloned
> into Bluescript. I sequence on a LI COR 4200 and get 700-1100 bp per
> sample. I have been using the LI COR AlignIR program to assemble and
> have crashed the program. I was told to put the contigs into a new
> project to save space, but at this point (85 kb) I have a few 5 kb
> contigs, but most samples haven't matched up yet, so this isn't
> feasible. I have access to the McVector/AssemblyLIGN program (Mac
> version), but the company isn't able to tell me how many samples this
> program can work with or whether a project of this size is feasible.
> Is it feasible to assemble a project this large on a personal computer?
> Do you think the McVector/AssemblyLIGN program would work? Do you think
> some of the other assembly packages out there would work (such as
> Sequencher?) If so, who is the vendor and what is the cost?
> Do I need to go to a Unix Platform? I would have access to a UNIX
> system (Phap/Phred) for about $500.00 per year.
> Any comments or suggestions would be greatly appreciated. Thank you in
> Sharon Minnerath
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