recalcitrant DNA

Chris Spolsky spolsky at
Mon Oct 23 17:02:49 EST 2000

I have been trying to digest some mitochondrial DNAs with a number of
restriction enzymes, to no avail. Control DNA digests OK, so the enzymes'
activity is not a problem. These are mtDNAs that previously (about 10 years
ago) were easily digested by the same restriction enzymes. Obviously
something has happened to the DNA on storage (-80, in 0.1X TE, pH8.0).

Does anybody have any idea what could have happened to the DNA, and what
can be done now to get it digested? Also, since it appears that longterm
storage in aqueous solution is suboptimal, how should mtDNA be stored for
the long term? 

Chris Spolsky


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