sadams at northnet.org
Wed Sep 27 09:45:41 EST 2000
I have used Sequencher for the past 5 or 6 years and find it to be
the fastest, easiest to use alignment software. It can't be beat for speed
and simplicity. I believe if you go the webpage for GeneCodes
http://www.genecodes.com/, you can download a demo copy for a Mac or send
away for a PC version. The Mac version is considerably cheaper than the PC
version. I have worked with the people at Genecodes extensively and their
tech support is great ( although it is so intuitive, you rarely need them).
I would at least check out Sequencher before making any decisions.
>I have a question about Alignment Software. I am currently sequencing a
>BAC which I am told is about 340 kb. I have it nebulized and subcloned
>into Bluescript. I sequence on a LI COR 4200 and get 700-1100 bp per
>sample. I have been using the LI COR AlignIR program to assemble and
>have crashed the program. I was told to put the contigs into a new
>project to save space, but at this point (85 kb) I have a few 5 kb
>contigs, but most samples haven't matched up yet, so this isn't
>feasible. I have access to the McVector/AssemblyLIGN program (Mac
>version), but the company isn't able to tell me how many samples this
>program can work with or whether a project of this size is feasible.
>Is it feasible to assemble a project this large on a personal computer?
>Do you think the McVector/AssemblyLIGN program would work? Do you think
>some of the other assembly packages out there would work (such as
>Sequencher?) If so, who is the vendor and what is the cost?
>Do I need to go to a Unix Platform? I would have access to a UNIX
>system (Phap/Phred) for about $500.00 per year.
>Any comments or suggestions would be greatly appreciated. Thank you in
Scottie Adams aka Pamela Scott Adams
ABRF Fragment Analysis Research Group
Molecular Biology Core Facility
Saranac Lake, NY 12983
Email: sadams at northnet.org
More information about the Autoseq