genotyping: DNA in PCR

[Scott Selby]

We have routinely used 10ng in either a 5 or 10ul reaction successfully.

Scott Selby, Scientist
Univ. of MN/Dept. of Medicine
Div. of Rheumatic and Autoimmune Disease
312 Church St. SE
6-244 BSBE
Minneapolis, MN  55455

Lynn Petukhova wrote:

> Hello everyone:
>
> I have a quick question for people doing Genescan:
>
> How much DNA do you use in your PCR?
>
> We use 20ng in a 5uL reaction and I would like to reduce this.  Thanks in
> advance for your feedback.
>
> Lynn
>
> Lynn Petukhova
> Assistant Director
> Genotyping Core Facility
> Starr Center for Human Genetics
> The Rockefeller University
> 1230 York Avenue, Box 241
> New York, NY  10021
>   phone: (212) 327-7181
>   fax:   (212) 327-7182
>
> ---

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