ABI TSR reagent sample loading on ABI310

Nick van Holst nick.vanholst at flinders.edu.au
Wed Apr 11 23:12:58 EST 2001


Does anybody know either :- 

the formulation for TSR reagent used for making up reactions prior to loading onto the 310

or

An alternative to TSR, I note that many people are using water/edta/formamide combinations for use on the 3700 would this be suitable for the 310.

all contributions welcome.

NB i am primarily sequencing PCR products up to ~1kb, with Exo1/SAP cleanup+Isopropanol ppt'n prior to addition of sample loading buffer (TSR)

Regards Nick van Holst
Research Associate
Centre for Digestive Health 
Department of Gastroenterology
Flinders Medical Centre 
Bedford Park 
Adelaide S.A. 5042
Australia




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