ABI TSR reagent sample loading on ABI310
Nick van Holst
nick.vanholst at flinders.edu.au
Wed Apr 11 23:12:58 EST 2001
Does anybody know either :-
the formulation for TSR reagent used for making up reactions prior to loading onto the 310
An alternative to TSR, I note that many people are using water/edta/formamide combinations for use on the 3700 would this be suitable for the 310.
all contributions welcome.
NB i am primarily sequencing PCR products up to ~1kb, with Exo1/SAP cleanup+Isopropanol ppt'n prior to addition of sample loading buffer (TSR)
Regards Nick van Holst
Centre for Digestive Health
Department of Gastroenterology
Flinders Medical Centre
Adelaide S.A. 5042
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