Big Dye v3.0 Dilutions?

Dennis Shubitowski shubitow at cvm.msu.edu
Thu Dec 20 15:51:34 EST 2001


Is anyone running diluted protocols using the Big Dye 3.0 kit? I am doing small scale PCR based sequencing and have only seen protocols for earlier versions of diltutions for the Big Dye chemistries. The 1X protocol seems like a large waste of terminator for the price.

Thank you!

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