ABI 3100: red dye blob at 240 basecalls

Roland.Varecka at vie.boehringer-ingelheim.com Roland.Varecka at vie.boehringer-ingelheim.com
Tue Jul 3 03:02:17 EST 2001


Dear Angela, dear Steve,

thanks for your hints on loading the multiscreen plates. I ran some test
yesterday
and the trend is clear: either reducing the loading volume from 30uL to 15u=
L
(plus
making sure the samples are applied exactly at center column) or increasing
the amount
of Sephadex G-50 from 45uL to 90uL produced very nice and clean sequences.=
=20
The latter method gave more consistent sample quality and samples are more
easily
loaded when using an 8-channel pipettor, however may be a bit messy for
routine application,
as the swollen gel slightly protrudes over the rim of the wells (reminded m=
e
a bit of freshly tapped draft beers). I have also ordered the Quiagen DyeEx
96 kit.

Thanks again,

Sincerly

Roland

---------------------------------------------------------------
Roland M Varecka
Boehringer Ingelheim Austria GmbH
Dr. Boehringergasse 5-11
A-1121 Wien, =D6sterreich

Tel:=09xx43-1-80105-2392
Fax:=09xx43-1-80105-2360
Email:=09roland.varecka at vie.boehringer-ingelheim.com

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