PE biosystems version 3 sequencing kit

Dr.Duncan Clark news at [127.0.0.1]
Thu Jul 19 10:02:44 EST 2001


In article <9j6ff2$q6t$1 at mercury.hgmp.mrc.ac.uk>, the eminent Joanne
Gilfillan at BIOSCI/MRC Human Genome Mapping Project Resource Centre
wrote
>We recently tried out the Big Dye Terminator Kit Version 3 on our ABI 
>377 sequencer.  We experienced an offset/shift of the GTP in relation 
>to the other bases, creating problems with the base calling. Version 
>1 of this kit gave us no problems at all. Has anyone else also found 
>this, and if so, are there any ideas on how to remedy this problem? 
>Does anyone know whether version3 has been tested extensively on 
>gel-based systems? 

Just a thought. Do you not need to generate a new matrix for the kit,
which will then overcome these problems?

Duncan
-- 
It might look like I'm doing nothing, but at the cellular level I'm really
quite busy.

Duncan Clark
GeneSys Ltd.
Tel. +44 (0) 1252376288
FAX +44 (0) 8701640382

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