Sequencing directly from BACs
sadams at aldus.northnet.org
Mon Jul 23 10:13:32 EST 2001
Dear Tonto. (I've heard the joke :-))
Try http://www.abrf.org/ and look under Research Groups/ DNA
sequence. Look for their 200/2001 study on BACs.
You will find a sample protocol which should work fine for you.
At 3:35 AM +0100 7/22/01, TONTO wrote:
>Anyone have any tricks for sequencing directly from BAC clones?
>Our core lab has never done it and they would like some input before they do
>some runs for us.
>I believe they are using the big dye terminator kit and a capillary
>sequencing machine. I can get more specific info if anyone has had luck
>tontogoldstein at hotmail.com
Scottie Adams aka Pamela Scott Adams
Molecular Biology Core Facility
100 Algonquin Avenue
Saranac Lake, NY 12983
Phone: 518-891-3080, Ext. 115
Email: sadams at northnet.org
sadams at trudeauinstitute.org
More information about the Autoseq