3700 Wizard problems

Phillip San Miguel pmiguel at purdue.edu
Tue Jul 31 08:45:56 EST 2001


SMarsh3807 wrote:

> >When changing arrays the Wizard program didn't complete the full operational
> >function , no acid wash occured.
> >Has any other user experienced this problem when using these Software
> >problems.
>
> We see this frequently as well. The best thing to do is reboot the 3700 before
> you start the wizard. Ours usually just kinda stops in the middle, or errors
> out. Rebooting the 3700 usually works for me.
> Steve Marsh
> Director DNA Seq Core Facility @ Caltech

    This could be similar to the polymer change wizard erroring out immediately
after starting it. I've notice this almost always happens if it is run with Data
Extractor open. If I close Data Extractor and Data Collection. Then reopen Data
Collection and run the polymer change wizard, I never have this error.
    My problems with regenerations seemed to be caused by a bum polymer pump my
machine was shipped with. We nursed it through about 1000 runs before our service
engineer took mercy on us and replaced it. Since then, no problems. Also I usually
check the hyperterminal log files. I could see we were getting pump overload
errors.

Phillip SanMiguel
Purdue Genomics Core Facility


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