Bi Sulphide Sequencing

Kevin Clark kevin at hammer.imm.ox.ac.uk
Thu Mar 22 11:46:49 EST 2001


Hi,

Is anyone running Bisulphide processed sequences on 377's?

We've just started to do this (with BigDye terminators) and find that
where the C's have been replaced by T's the sequence shows a small C
peak under the T but it is out of line with the T, arriving about half a
base later.

Any idea what's happening? Is the software over compensating for lack of
C signal in the data and bringing up C's from the background? Would dye
primer sequencing cure it? Has anyone been able to reproducible quantify
the peak heights?

Thanks in advance.

Kevin Clark
IMM 
Oxford
UK

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