genotyping: DNA in PCR

Lynn Petukhova orvietl at
Wed Mar 28 10:14:53 EST 2001

Hello everyone:

I have a quick question for people doing Genescan:

How much DNA do you use in your PCR?

We use 20ng in a 5uL reaction and I would like to reduce this.  Thanks in
advance for your feedback.


Lynn Petukhova
Assistant Director
Genotyping Core Facility
Starr Center for Human Genetics
The Rockefeller University
1230 York Avenue, Box 241
New York, NY  10021
  phone: (212) 327-7181
  fax:   (212) 327-7182  


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