Separating mixed sequence traces?

Duncan Clark d_clark at genesys.demon.co.uk
Mon Nov 5 11:25:08 EST 2001


In message <9s5u60$4ke$1 at mercury.hgmp.mrc.ac.uk>, Kevin Clark 
<kevin at hammer.imm.ox.ac.uk> writes on the Mon, 5 Nov 2001
>Is there any software that allows you to separate two sequences from a
>mixed template reaction?

Well think about it. How on earth are you going to be able to tell which 
base belongs to which sequence in the mixture at any one position.

Imagine you could get an A and C then an  A and T in the next position.

Software could assign AA, AT, CA or CT. Keep doing this at every 
position and you will have hundreds of possible sequence combinations. 
If you know the sequence of one of the mix DNA's then it will help, 
otherwise I would start again.

Duncan

-- 
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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