Separating mixed sequence traces?
kevin at hammer.imm.ox.ac.uk
Tue Nov 6 05:25:54 EST 2001
Duncan Clark wrote:
> In message <9s5u60$4ke$1 at mercury.hgmp.mrc.ac.uk>, Kevin Clark
> <kevin at hammer.imm.ox.ac.uk> writes on the Mon, 5 Nov 2001
> >Is there any software that allows you to separate two sequences from a
> >mixed template reaction?
> Well think about it. How on earth are you going to be able to tell which
> base belongs to which sequence in the mixture at any one position.
> Imagine you could get an A and C then an A and T in the next position.
> Software could assign AA, AT, CA or CT. Keep doing this at every
> position and you will have hundreds of possible sequence combinations.
> If you know the sequence of one of the mix DNA's then it will help,
> otherwise I would start again.
I understand that but, as I stated in the original post, I was just
relaying a question from a colleague.
There is supposedly software that does this. She had read about it in a
paper. As you say, presumably it uses a reference trace from a known
sequence and subtracts that to leave the other trace.
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