BigDye version 3 vs version 1

Mark Pickett map at soton.ac.uk
Thu Nov 8 05:39:37 EST 2001


Just how different are the BigDye version 1 and version 3 chemistries? In
our labs (using a 377 plate sequencer) the version 3 seems to be failing
where the version 1 gave good results (PCR products/plasmid templates). Is
it possible to dilute version 3 in a similar way to version 1?
Using the Promega "Wizard Magnesil Sequencing Clean Up System" worked ok for
version 1 but we see a distinct loss of product (esp. smaller fragments
<100bp) with version 3.
Has anybody had similar results?
Mark Pickett
University of Southampton







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