KS primer quality in high throughput sequencing
jkhattra at bcgsc.bc.ca
Mon Nov 26 18:38:48 EST 2001
Seeking opinions on the quality of sequence obtained with the KS primer (TCGAGGTCGACGGTAT) using Dye Terminator cycle sequencing chemistry and capillary electrophoresis. My specific situation involves pPCR-Script Cam SK(+) vector with blunt-ended microbial genomic DNA inserts.
Genome Sequence Centre
BC Cancer Agency
600 West 10th Ave.
Vancouver BC V5Z4E6
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