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Jeff Wong sornamjeff at yahoo.com
Wed Oct 31 07:51:16 EST 2001


Hello,

I am performing DNA Sequencing of methylated bases on
my ABI 377. I got phenomena of signals shooting up
where no particular base present (e.g C or G) and
having target bases shifted.  I've asked ABI for
advice, ending up adjusting the basecaller's rfu ratio
setting and no further suggestion came out. However,
it seems that I still don't get any well optimised
electropherogram results. 

Would there be anyone who has done bisulphite modified
DNA sequencing and could comment on optimising this
kind of research work?

Jeff


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