ABI kit Genotyping PCR help
radomski at interchange.ubc.ca
Tue Sep 4 16:29:51 EST 2001
While carrying out genotyping using ABI's LMS-MD10 kit using MJ Tetrads
for the thermocycling, we have noticed some data quality/failure rate
issues that we think might be related to using 96-well PCR heads versus
For the 96-well format we use 5 degree offset lid tracking. And for
384-well we use 80 degree constant. For the thermocycling parameters, we
use the standard ABI protocol.
Before changing over to 384-well format, we spoke to MJ tech. support and
they said that the only parameter we should have to change is the lid
Now with our current problems, and a subsequent call to tech. support,
they suggested decreasing the denaturation temperature.
So, before starting to optimized this and other parameters, does anyone
have any hints to offer?
More information about the Autoseq