Amersham kit on 3100

inagy at abc.hu inagy at abc.hu
Wed Apr 24 13:21:57 EST 2002


We have remainders from old Amersham Thermo Sequenase dye terminator cycle
sequencing kits (US 79865).
When we tried to use reactinos from this kits on an ABI 3100, the
chromatograms looked nice, but the base calling was not correct.
I guess, it was due to the improper dye standard set.
However, there is no information in the kit protocol about the dyes,
or about the proper settings on ABI capillary machines.

I am wondering, whether somebody knows this.

Regards

Istvan

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