Dye Cleanup and Big Dye ver. 3?

Dr N.I.Leaves nleaves at hgmp.mrc.ac.uk
Thu Apr 25 05:31:23 EST 2002


Hi Mick,

we use a 384 well bind plate product from Millipore for 3700 big dye clean
ups. The product code is S384SEQ50. They are quite expensive but we have
integrated them into our robotic system in a way which we could not do
with EtOH precipitation. Also, we find them more reliable in 384 well
clean ups than EtOH.

We have just moved to BD3 and find signal intensity to be very strong. The
Millipore plates are OK with BD and BD2. Do you use all 384 plates? - if
so you are limited to a few products.

Good luck

nick

end
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Dr N I Leaves
Mouse Sequencing
MRC HGMP Resource Centre
Hinxton
Cambridge CB10 1SB
tel: 01223 494557 (office) or 01223 494541 (lab)
email: nleaves at hgmp.mrc.ac.uk
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