DNASeq - MegaBACE matrix
hall at RUBICONGENOMICS.com
Mon Feb 18 10:32:12 EST 2002
I posed this very same question to Amersham.
The answer is you cannot because you run a serious risk of introducing
air bubbles by injecting 'spent' LPA matrix
From: Glenis Wiebe [mailto:wiebe at mpi-cbg.de]
Sent: Saturday, February 16, 2002 10:06 AM
To: ABRF Newsgroup; autoseq at net.bio.net
Subject: DNASeq - MegaBACE matrix
I would like to hear from MegaBACE users regarding matrix usage.
Specifically, I am interested to know if anyone re-uses the matrix that
is left in the pre-filled tubes after a run, and if so:
1) Can the volume be below the recommended volume of 0.7 ml?
2) Can small amounts be combined together for re-use?
3) How many times can this be done?
Thanks in advance
Facility Leader, DNA Sequencing
Max Planck Institute of Molecular Cell Biology and Genetics
e-mail: wiebe at mpi-cbg.de
The views and ideas posted on the ABRF Electronic Discussion List are
those of the participants and no responsibility can be taken by the
To unsubscribe yourself from this mailing list, send a blank email to:
<list_leave_ABRF at list.abrf.org>
To change the address at which you receive the ABRF List just
"unsubscribe" using the old email account and "subscribe" using the new
To post anonymously, send a message to ombudsmn at aecom.yu.edu (note
Please do not set "autoresponders" to reply to this list. A better
solution is to "suspend" your subscription when you leave and
"unsuspend" when you return.
For these and other list commands, send a blank email to:
<list_help_ABRF at list.abrf.org>
For posting guidelines and other information see our web site at
For questions and problems, please send a message to
listmaster at list.abrf.org
You are currently subscribed as: hall at rubicongenomics.com
More information about the Autoseq