Storing diluted BigDye premixes.

David C.Fritzinger dfritzin at nospam.hotmail.nospam.com
Thu Jun 13 23:25:17 EST 2002


In article <ae9lbn$1pr$1 at mercury.hgmp.mrc.ac.uk>, Martin Hirst
<hirst at interomex.com> wrote:

> Good Day,
>
> Does anyone have any experience with storing "premixes" of diluted bigdye?
> Is it possible to create a large batch of a premix (ie. Bigdye, primer and
> water) aliquoted into 96 well plates and stored at 4C or is it imperative
> that the premix is made fresh just prior to template addition?
>
>
>
> Cheers
>
> Martin

I've stored big dye primer premixes, aliquoted into plates, at -20 deg
for weeks, and they've worked well.

Dave Fritzinger
>
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>
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>
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