Diluting DYEnamic ET Terminator Cycle Seq for use in MegaBase

Bruce A.Roe broe at ou.edu
Thu Mar 7 09:44:12 EST 2002


Tom, et al.

We could reduce the volumes by 1/4th as described on our web site at URL:

        http://www.genome.ou.edu/ET_Terminators.html
but were not that successfull with going any further.  However, I recall
that
because Amersham's price was ~1/4th that of ABI's kits, the 1/4 reduction

in volume used made both competitive since we were using 1/16th of the
BigDye Version 1.

If folks have not seen it yet, we now are at 1:20 routinely with ABI's
Big
Dye Version 3 and the protocol we are using is at URL:
    http://www.genome.ou.edu/PredispensedSeqMixBDv3.html

I've also updated several other protocols that can be accessed from our
protocol
page at URL:
    http://www.genome.ou.edu/proto.html

Cheers,
--bruce

Tom wrote:

> Has anyone determined whether the DYEnamic ET Terminator Cycle Seq can
> be diluted for use in MegaBase instruments?  I'm thinking about the
> DYEnamic ET Terminator Dilution Buffer.
>
> Tom.
>
> ---

--
*************************************************************************

Bruce A. Roe, Ph.D  George Lynn Cross Research Professor
                    Department of Chemistry and Biochemistry
                    University of Oklahoma, Norman, OK 73019-0370, U.S.A.

Phone: (405) 325-4912 or 7610;  FAX: (405) 325-7762;  e-mail: broe at ou.edu

********************** http://www.genome.ou.edu/ ************************



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