Tom, et al.
We could reduce the volumes by 1/4th as described on our web site at URL:
http://www.genome.ou.edu/ET_Terminators.html
but were not that successfull with going any further. However, I recall
that
because Amersham's price was ~1/4th that of ABI's kits, the 1/4 reduction
in volume used made both competitive since we were using 1/16th of the
BigDye Version 1.
If folks have not seen it yet, we now are at 1:20 routinely with ABI's
Big
Dye Version 3 and the protocol we are using is at URL:
http://www.genome.ou.edu/PredispensedSeqMixBDv3.html
I've also updated several other protocols that can be accessed from our
protocol
page at URL:
http://www.genome.ou.edu/proto.html
Cheers,
--bruce
Tom wrote:
> Has anyone determined whether the DYEnamic ET Terminator Cycle Seq can
> be diluted for use in MegaBase instruments? I'm thinking about the
> DYEnamic ET Terminator Dilution Buffer.
>> Tom.
>> ---
--
*************************************************************************
Bruce A. Roe, Ph.D George Lynn Cross Research Professor
Department of Chemistry and Biochemistry
University of Oklahoma, Norman, OK 73019-0370, U.S.A.
Phone: (405) 325-4912 or 7610; FAX: (405) 325-7762; e-mail: broe at ou.edu
********************** http://www.genome.ou.edu/ ************************
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