Tailing of C and G peaks

Jean Renaud jean.renaud at rsvs.ulaval.ca
Tue Mar 12 11:24:41 EST 2002


Bonjour =E0 tous,

What causes tailing of C and G peaks (BigDye v. 3.0 chemistry) and how do I
prevent it ? Tailing is mainly observed with sequencing of PCR products. We
didn't observe tailing when we used BigDye v. 1.0 chemistry.

Cordiales Salutations,
Jean

Jean Renaud

Responsable scientifique
Laboratoire de Synth=E8se et d'Analyse d'Acides Nucl=E9iques
Recherche en Sciences de la Vie et de la Sant=E9
Pavillon Charles-Eug=E8ne Marchand
Local 3233
Universit=E9 Laval
Ste-Foy, (Qu=E9bec)
Canada
G1k 7P4
T=E9l. : 418-656-2552
Courrier =E9lectronique : jean.renaud at rsvs.ulaval.ca


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