Need: protocol for direct sequencing of PCR product

Spence, Koren R SpenceK at mar.dfo-mpo.gc.ca
Wed Mar 20 15:36:10 EST 2002


Does anyone know where I can find a protocol for direct sequencing from PCR
product?

We have been trying (unsuccessfully) to sequence PCR product on our MJ
BaseStation.  We have been using Qiagen PCR cleanup kits and Sequencing
reaction clean up kits, but even still, nothing is working.  Any information
or advice would be greatly appreciated!

Thank you.
Koren Spence
Molecular Biologist
Bedford Institute of Oceanography

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