Need: protocol for direct sequencing of PCR product

Bruce Roe broe at ou.edu
Sat Mar 23 10:12:22 EST 2002


Koren, et al,
	We have several protocols for direct sequencing from PCR
products, on our protocol web page at URL:

	http://www.genome.ou.edu/proto.html

Specifically, look at:

	http://www.genome.ou.edu/uniplexPCRbasedseq.html
Cheers,
--b.roe

Spence, Koren R wrote:

> Does anyone know where I can find a protocol for direct sequencing from PCR
> product?
>
> We have been trying (unsuccessfully) to sequence PCR product on our MJ
> BaseStation.  We have been using Qiagen PCR cleanup kits and Sequencing
> reaction clean up kits, but even still, nothing is working.  Any information
> or advice would be greatly appreciated!
>
> Thank you.
> Koren Spence
> Molecular Biologist
> Bedford Institute of Oceanography
>
> ---
>
>


-- 
*************************************************************************
Bruce A. Roe, Ph.D  George Lynn Cross Research Professor
                     Department of Chemistry and Biochemistry
                     University of Oklahoma, Norman, OK 73019-0370, U.S.A.
Phone: (405) 325-4912 or 7610;  FAX: (405) 325-7762;  e-mail: broe at ou.edu
********************** http://www.genome.ou.edu/ ************************

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