BigDye v3.0 dilution?
eapeden at ucdavis.edu
Tue May 14 16:01:11 EST 2002
I do a small amount of sequencing from PCR products until now I
have been using applied biosystems Big Dye v2.0. It seems I will have to
get Big Dye v3.0. I was wondering if I would be able to dilute the newer
chemistry the same way I was diluting before. Currently I use 1ul of the
BigDye Mix, 2ul of a dilution buffer, 0.5ul of 10uM sequencing primer, and
3.5ul of PCR product. Any thoughts would be greatly appreciated. Thanks.
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