problems with sequencing in big dye v3

SMarsh3807 smarsh3807 at aol.com
Sun Oct 13 23:57:29 EST 2002


>10mM tris, 1mM tris ou 0.1 mM tris with no difference...

We have been finding that any buffer in the template has been cuasing us
problems with bd v3.0. The new v3.1 seems to have helped some, but we are still
seeing some problems. Having the template and primer only in MilliQ water with
no tris whatsoever does help a lot.
Steve
DNA Sequencing Core @ Caltech
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