Sequencing Very AT Rich sequences on a ABI 3700 with BigDye

SMarsh3807 smarsh3807 at
Sun Oct 13 23:59:32 EST 2002

We have found that version 3.0 and v 3.1 really help with the difficult
templates like AT and GC rich. We do run them with higher denaturation
temperatures and a 5 min hot start at 95 degrees C. Hope this helps.
DNA Sequencing Cor @ Caltech

More information about the Autoseq mailing list