Large "G" Peak ~85 bases

John jws36065 at GlaxoWellcome.com
Tue Sep 10 10:31:31 EST 2002


We are using dye terminator chemistry on the ABI Prism 3700.
The "G" blob is the only one we are seeing - no "A," "T," or "C" - so,
we're certain our purification method isn't to blame.
Any other suggestions would be great.


> I think you need to give some more information. What kind of sequencer
> are you using. What kind of chemistry are you using?
>
> I'll make a stab at it, but since I don't know the chemistry you're
> using, I could be wrong. Assuming that you are using dye terminator
> chemistry, the obvious possibility is that you aren't completely
> removing the terminators from your reaction. If that is the case, you
> might want to try a better method of cleaning up your reactions. One
> possibility would be to run them over special spin columns designed
> just to remove excess terminators.
>
> David C. Fritzinger, Ph.D.
> Cancer Research Center of Hawaii
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>
>
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