3700 Data Collection v2 extractor can invisibly delete

Phillip San Miguel pmiguel at purdue.edu
Wed May 14 13:52:48 EST 2003


Phillip San Miguel wrote:

>    Just a "heads-up" for those using Data Collection v. 2 on the 3700
>for sequencing. While a great improvement over v 1.1 in many ways, it
>does appear to have one characteristic that I find extremely
>undesirable--even frightening. [...]
>    To spell out why this is bad, the result is that sequences generated
>will have 20-30 base deletions every 150-200 bases (in my case). You
>won't see anything odd by looking at the chromatograms--only by paging
>through a run in Data Collection will one clearly see blank areas.
>    [...]
>
    To follow up: my laser has been replaced. But now Applied Biosystems
is saying that the "block dropping" phenomenon probably has nothing to
do with the laser. Rather version 2 of data collection can start to drop
blocks in the way I describe above if the sequencer and computer are not
restarted every 2-3 days!
    Given that version 2 is set-up to allow continuous running (rolling
loading) this seems most unfortunate. Restarting the sequencer wastes
all the polymer that is in the sheath flow shringe.
    Has anyone seen this phenomenon?
Phillip SanMiguel
Purdue Genomics Core Facility

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