3700 Data Collection v2 extractor can invisibly delete

Phillip SanMiguel pmiguel at purdue.edu
Fri May 16 15:30:34 EST 2003



Dr N.I. Leaves wrote:
>>    To follow up: my laser has been replaced. But now Applied Biosystems
>>is saying that the "block dropping" phenomenon probably has nothing to
>>do with the laser. Rather version 2 of data collection can start to drop
>>blocks in the way I describe above if the sequencer and computer are not
>>restarted every 2-3 days!
>>    Given that version 2 is set-up to allow continuous running (rolling
>>loading) this seems most unfortunate. Restarting the sequencer wastes
>>all the polymer that is in the sheath flow shringe.
>>    Has anyone seen this phenomenon?
>>Phillip SanMiguel
>>Purdue Genomics Core Facility
>>
>
>
> Hi Phillip,
>
> our three 3700 machines are run pretty much constantly, I reckon we
> restart the computer and sequencer about once every two weeks. I have
> never seen the dropping out you describe.
>
> nick
>
>[...]
Thanks for the response, Nick.

Are you running version 2.0 of Data Collection?
Applied Biosystems is now saying that restarting once a week should be
sufficient.

Phillip SanMiguel

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