Low voltage on LI-COR sequencer, why?
Yong Mie Lee
AngelaYong at bst-asia.com
Wed Oct 15 03:16:25 EST 2003
I finally solved the low voltage problem for the LI-COR 4200 DNA sequencer in our lab. First of all, I noticed that there are several recipe of TBE buffer available for acrylamide & agarose gel electrophoresis.
I use the recipe below: -
(i) For 20x TBE:
Boric acid 61.7g
Top up to 1 liter with dH2O and autoclave to complete dissolved the salt.
(Some precipitation would form after a period, but still can perform well)
Another recipe used that do not caused precipitation were: -
(ii) For 20x TBE:
Boric acid 110g
0.5M EDTA (pH8) 80mL
Top up to 1 liter
Please note that the chemical used in (ii) is almost double and the form of EDTA added is different. I'm also not sure why it caused a big different towards our sequencer used. Somehow deionised water would not bring such a big different on voltage detected. For recipe (i) can bring up and maintain the maximum voltage (1500V for short gel and 3000V to long gel) on LI-COR DNA sequencer.
I love to share this with you. Hopefully some of you could discuss further.
Angela Yong Mie Lee
More information about the Autoseq