[Automated-sequencing] Sanger sequencing obsolete?
pmiguel at purdue.edu
Tue Jun 21 20:06:26 EST 2005
Not for a while anyway. But I'm getting the impression that some of the
$100,000 and $1000 human genome sequence methodologies are beginning to
Anyone plunked down half a million dollars for a 454 Life Sciences
Well, that web page only really works with Internet Explorer. But the
take home is that it is a sequencer that does about 200,000 reads per
run. But median read length is 100 bases. Library construction needs to
be on beads that are centrifuged into individual wells of a 1.6-million
well "pico titer plate". The actually sequencing is done via
synthesis/pyrosequencing/luciferase reactions. As each base is added (if
one is added) light is emitted and the machine somehow detects the light
and the pico titer plate position of where the light is emitted.
When I heard about this technology, I searched the web and found the
But it might be a little out of date. Basically the take-home from that
Location: Essex, UK.
Founded: Spun out of the University of Cambridge
Technology: Single-molecule sequencing utilizing
DNA cluster technology. Arrays will be
capable of sequencing 100 million sample
DNA templates per cm2 of chip.
Read Length: 25-30 bases.
Expected Launch: Expects to be sequencing
genomes next year.
Location: Cambridge, Mass.
Founded: May 2003.
Technology: Single-molecule sequencing in
which up to 300 million fragments can be
attached to a single slide, enabling 10x
coverage of the human genome in a single
Read Length: 5-10 bases now, but working
on increasing that to 25 bases.
Expected Launch: Undisclosed.
Location: Lübeck, Germany.
Founded: Spun out of the University of Lübeck
in May 2002.
Technology: AnyGene technology utilizing
the sequencing-by- synthesis method.
Read Length: 45 nucleotides, but with a 30%
Expected Launch: Aiming for 2006.
454 Life Sciences
Location: Branford, Conn.
Founded: Spun out of Curagen in 2000.
Technology: Sequencing-by- synthesis method
whereby beads are attached to individual
strands of DNA and placed in individual wells
of its PicoTiterPlate.
Read Length: Short readlengths. Company researchers
have achieved production
readlengths of 100 bases.
Expected Launch: Q1 2005.
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