[Automated-sequencing] Weird chromat, part 2

[Jean Renaud]

The solution to obtain good chromatogram is to 
increase dilution with formamide in order to 
decrease viscosity of sample.
Jean


Hi Phillip,

Sometimes, presence of genomic DNA in the prep 
may give some viscosity to it and cause trailing 
of peaks. Effect is more important as molecular 
weight of fragments increase.

Jean


>Take a look at this screen capture:
>
>http://data.genomics.purdue.edu/~pmiguel/technical/06-22-2005read.htm
>
>
>(It is only a 74 kbyte .png file.)
>
>Anyone have an idea of what is going on? Note 
>that the peaks look pretty good around 200 
>bases, but by 500 bases something is clearly 
>wrong. The right-side edge of the peaks come up 
>pretty high above baseline. But the left-side 
>edge looks much better. I guess the peaks are 
>"skewed".
>
>Has anyone seen this before?
>
>--
>Phillip
>
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>Autoseq at net.bio.net
>http://www.bio.net/biomail/listinfo/autoseq


-- 
Jean Renaud

Responsable scientifique
Laboratoire d'analyse et de synthèse d'acides nucléiques
Recherche en Sciences de la Vie et de la Santé
Pavillon Charles-Eugène Marchand
Local 3233
Université Laval
Ste-Foy, (Québec)
Canada
G1K 7P4
Tél. : 418-656-2003
          418-656-2552
Fax. : 418-656-7176
Courrier électronique : jean.renaud at rsvs.ulaval.ca

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