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[Automated-sequencing] RE: [SPAM] - Autoseq Digest, Vol 6, Issue 3 - Email found in subject

Fulton, Ciaran ciaran.Fulton at arradx-almac.com
Thu Nov 17 11:24:20 EST 2005

Hi Philip...ironically we were just discussing the same error message that occurred on our 48-cap (3730) machine a week ago. This happened after the first half of the plate had been processed. The water tray had moved under the array and the machine just hung after generating this error message which was rather bizarre given that it was processing the same plate. No instrument warning light was observed and the error hasn't occurred subsequently. I'm sorry I can't shed anymore light on the matter Philip, nor have ABI technical support (I'm still waiting for them to return my call).


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Today's Topics:

   1. DNA SEQ: 3730XL Tray on deck error (Phillip San Miguel)


Message: 1
Date: Tue, 15 Nov 2005 13:45:18 -0500
From: Phillip San Miguel <pmiguel at purdue.edu>
Subject: [Automated-sequencing] DNA SEQ: 3730XL Tray on deck error
To: bionet-genome-autosequencing at moderators.isc.org
Message-ID: <437A2CBE.4090708 at purdue.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

We keep running into a maddening error on our 3730XL:

Tray on deck does not match tray type in run setup

Maddening because this error usually happens in the middle of a batch of 
runs from a 384-well plate. That is, the first or first few quads get 
loaded and run fine. Then the batch errors out.

Usually it requires a service call to get the machine running again. But 
I'm not entirely sure that the service call is what "fixes" the problem. 
It might be turning the machine off for a couple of days. (An hour isn't 
long enough. I just tried it.)

Replacing the plate sensor is among the methods that gets us running 
again--for a time. But looking through old 3730.log files I can see 4 
times that this has shut us down for a couple of days since the middle 
of the summer.

Last time it coincided with our laser going out. The laser was replaced 
and we ran for four days. Only to see this error pop up again.

Any ideas?


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