[Automated-sequencing] Re: Demand of informations

Phillip San Miguel pmiguel at purdue.edu
Mon Feb 27 09:41:01 EST 2006


attaoua at montp.inserm.fr wrote:
> Sir,
> I've heard that you have developed a technique concerning the sequencing
> of long DNA fragments. Can you please send me the protocole because it
> will help me in manipulations.
>  Sincerly yours.
>            Redha ATTAOUA
>            PhD student
>       Molecular Endocrinology Laboratory
>          IURC -Montpellier- France
> 
> 

The standard sequencing technique works independent of template length. 
It is (somewhat) dependent on template concentration.

What is the length of your template? What type of sequencing are you 
doing? Will you run your sequence reactions on an automated sequencer or 
a gel you will read yourself?

Phillip SanMiguel
Purdue Genomics Core Facility



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