IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

[Automated-sequencing] Surfactant in 5xABI buffer?

Phillip San Miguel pmiguel at purdue.edu
Mon Mar 20 09:29:55 EST 2006


I noticed last week that when I shook a bottle of "5X" ABI buffer (a 
stock now supplied with all Big Dye 3.1 purchases), I saw bubbles that 
would suggest the presence of a surfactant. The canonical recipe for 
"5X" ABI buffer, see:

http://www.genome.ou.edu/big_dyes_plasmid.html

for example, includes no surfactant.

What do you think? Is there now a surfactant in "5X" ABI buffer?


Phillip SanMiguel
Purdue Genomics Core Facility



More information about the Autoseq mailing list

Send comments to us at biosci-help [At] net.bio.net