I noticed last week that when I shook a bottle of "5X" ABI buffer (a
stock now supplied with all Big Dye 3.1 purchases), I saw bubbles that
would suggest the presence of a surfactant. The canonical recipe for
"5X" ABI buffer, see:
for example, includes no surfactant.
What do you think? Is there now a surfactant in "5X" ABI buffer?
Purdue Genomics Core Facility